ALKBH2 protein (His tag) (80R-1773)
Purified recombinant Human ALKBH2 protein
Overview
Overview
Synonyms | Oxy DC1 protein, ALKBH-2 protein, ALKBH 2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 protein, ALKBH-2, ALKBH 2 protein, ALKBH2, ABH2 protein |
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Species | Human |
Protein Type | Recombinant |
Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of ALKBH2 protein (His tag) (80R-1773)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
Residues | 1-261 amino acids: MGSSHHHHHH SSGLVPRGSH MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K |
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Expression System | E.coli |
Purity | > 90% pure |
Molecular Weight | 31.4 kDa (281aa), confirmed by MALDI-TOF |
Tag/Conjugate | His tag |
Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 30% glycerol. |
Concentration | 0.5 mg/ml |
Storage & Safety
Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
Biological Significance | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and ?-ketoglutarate for enzymatic activity. Recombinant human ALKBH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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