Alphaenolase protein (30R-1404)
Purified recombinant Human Alphaenolase protein
|Synonyms||Enolase 1 protein, C myc promoter binding protein protein, 2 phospho D glycerate hydro lyase protein, Alpha enolase protein, Phosphopyruvate hydratase protein, MPB 1 protein, ENO1L1 protein, MYC promoter-binding protein 1 protein, Non neural enolase protein, Enolase 1 (alpha) like 1 protein, Non-Neuronal Enolase (NNE) protein, MYC promoter binding protein 1 protein, EC 188.8.131.52 protein, NNE protein, MBP-1 protein, PPH protein, Enolase-1 protein, , MBPB1 protein, PPH protein, Plasminogen binding protein protein, MBP 1 protein, ENO1 protein, Enolase 1 (alpha) protein, tau-crystallin protein, MPB1 protein, MBP1 protein|
Coomassie Blue stained SDS-PAGE of Alphaenolase protein (30R-1404)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-434 amino acids: MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK|
|Purity||> 90% pure|
|Molecular Weight||47.1 kDa (434aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1 mM MgSO4 and 10% glycerol.|
Usage & Assay Information
|Bioactivity||Specific activity: > 13 units/ml. One unit will convert 1.0 umole of 2-phosphoglycerate to phospho(enol)pyruvate per minute at pH 7.5 at 25C|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoprotein associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography.|
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