AMACR antibody (10R-1851)

Mouse monoclonal AMACR antibody

Synonyms Monoclonal AMACR antibody, Anti-AMACR antibody, alpha-Methylacyl-CoA Racemase antibody, P504S antibody
Cross Reactivity Human, Mouse
Applications ELISA, IF, IHC, WB
Immunogen AMACR antibody was raised in Mouse using a purified recombinant fragment of human AMACR expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using AMACR antibody (10R-1851) (AMACR antibody)

Confocal immunofluorescence analysis of LNCaP cells using AMACR antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 2A10F3
Isotype IgG2b
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance alpha-methylacyl-CoA racemase (AMACR) is an enzyme that is involved in peroxisomal beta-oxidation of dietary branched-chain fatty acids. Recent studies have shown that, compared with expression in normal or benign prostate epithelium, AMACR is consistently overexpressed in prostate cancer epithelium, making it a specific marker for cancer cells within the prostate gland.

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Images

  • Immunofluorescent staining using Western Blot analysis using AMACR antibody (10R-1851) (AMACR antibody) | Confocal immunofluorescence analysis of LNCaP cells using AMACR antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using AMACR antibody (10R-1851) | Western Blot showing AMACR antibody used against Jurkat (1) and LNCaP (2) cell lysate.
  • Immunohistochemical staining using AMACR antibody (10R-1851) | Immunohistochemical analysis of paraffin-embedded human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using AMACR antibody with DAB staining.
  • Immunohistochemical staining using AMACR antibody (10R-1851) | Immunohistochemical analysis of paraffin-embedded human brain cerebellum using AMACR antibody.

Availability: In stock

Price: $395.00
Size: 100 ul
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