Angiopoietin 1 antibody (20R-AR013)
Rabbit polyclonal Angiopoietin 1 antibody
|Synonyms||Polyclonal Angiopoietin 1 antibody, Anti-Angiopoietin 1 antibody, Angiopoietin 1, Angiopoietin -1, Angiopoietin -1 antibody, ANG1 antibody, KIAA0003 antibody, AGPT antibody, ANG 1 antibody, Angiopoietin1 antibody, AGP 1 antibody, ANGPT 1 antibody, ANGPT1 antibody, AGP1 antibody, Angiopoietin 1 antibody, Angiopoietin 1|
|Specificity||Murine angiopoietin 1|
|Immunogen||Angiopoietin 1 antibody was raised in rabbit using a synthetic peptide corresponding to a region near the N-terminus of mouse angiopoietin 1 protein conjugated to KLH as the immunogen.|
Western Blot analysis using Angiopoietin 1 antibody (20R-AR013)
Rabbit anti-Ang-1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells.
|Form & Buffer||0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:5,000-1:25,000, WB: 1:500-1:2,000|
Storage & Safety
|Storage||Store at -20 deg C. Aliquot and freeze at -20 deg C or below for long term storage. This product is stable for several weeks at 4 deg C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freeze/thaw cycles.|
|Biohazard Information||This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.|
|Biological Significance||Angiopoeitin-1 (Ang-1) and Angiopoietin-2 (Ang-2) have importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver.|
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