ANKRD54 protein (His tag) (80R-2827)
Purified recombinant ANKRD54 protein (His tag)
|Synonyms||ANKRD54, ANKRD 54, ANKRD-54, ANKRD54, ANKRD 54 protein, ANKRD-54 protein, Ankyrin repeat domain-containing 54 protein, LIAR protein, Ankyrin repeat domain containing 54 protein|
SDS-PAGE analysis of ANKRD54 protein (His tag) (80R-2827)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-300 aa: MGSSHHHHHH SSGLVPRGSH MGSMAAAAGD ADDEPRSGHS SSEGECAVAP EPLTDAEGLF SFADFGSALG GGGAGLSGRA SGGAQSPLRY LHVLWQQDAE PRDELRCKIP AGRLRRAARP HRRLGPTGKE VHALKRLRDS ANANDVETVQ QLLEDGADPC AADDKGRTAL HFASCNGNDQ IVQLLLDHGA DPNQRDGLGN TPLHLAACTN HVPVITTLLR GGARVDALDR AGRTPLHLAK SKLNILQEGH AQCLEAVRLE VKQIIHMLRE YLERLGQHEQ RERLDDLCTR LQMTSTKEQV DEVTDLLASF TSLSLQMQSM EKR|
|Purity||> 85% pure|
|Molecular Weight||34.9 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||ANKRD54, also known as LIAR, contains 4 ANK repeats. This protein plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation. This protein interacts (via ankyrin repeat region) with LYN (via SH3-domain) in an activation-independent status of LYN. It forms a multiprotein complex with LYN and HCLS1. ANKRD54 also interacts with TSN2, VAV1, DBNL AND LASP1. Recombinant human ANKRD54 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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