Phospho specific antibodies

Phosphospecific antibodies are polyclonal or monoclonal antibodies that are specific for a target protein that is phosphorylated on a specific tyrosine, threonine, or serine residue. Phosphospecific antibodies allow the user to determine the status of individual phosphorylation sites, which can indicate the active state of a protein, an extremely useful experimental tool. Additionally, antibodies directed to phosphothreonine, phosphoserine or phosphotyrosine can be used to indicate whether a protein is phosphorylated in response to different stimuli, useful for determining protein behaviour and function.

Phosphoproteins are considered to be among the most important proteins in the body. They are the proteins responsible for regulating nearly all cellular processes from cell division, immunology and cancer to signal transduction in learning and memory.

Fitzgerald Industries offers a wide range of phosphospecific antibodies in monoclonal and polyclonal formats from all commonly used host species. Our high quality phosphospecific antibodies are the perfect choice for use in a wide variety of immunodetection applications including immunohistochemistry, ELISA, Flow Cytometry, Immunostaining and more.

Some of our most popular phosphospecific antibodies include:

Phospho-p53 antibody: p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. This rabbit polyclonal p53 antibody is rated for use in Immunoprecipitation, Western Blotting and immunohistochemistry. The antibody is specific for phosphorylation of Ser15 of human p53. Below illustrates a Western blot analysis of extract from UV treated HeLa cells (Lane 2) versus untreated HeLa cells (Lane 1) where the phospho-p53 antibody detects the activated p53 which has been brought about by UV induced DNA damage to the cells.

Western blot analysis of extract from UV treated (Lane 2) and untreated (Lane 1) HeLa cells.

 

 

Check out our range of Tau antibodies and other Alzheimer's related antibodies in our special Neurodegnerative Disease Antibody brochure.

Phospho-Myc antibody: c-Myc is phosphorylated in vitro by MAP Kinase at Ser62 and in vivo c-Myc is phosphorylated at both Thr58 and Ser62. Mutation of The58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription, suggesting that phosphorylation regulates the ability of c-Myc to stimulate transcription. This Phospho-cMyc antibody also gives excellent results in Immunoprecipitation, Western blotting and immunohistochemistry. It reacts with human, rat and mouse cMyc which is phosphorylated at Thr58/Ser62.

Phospho-Rb (SER780) antibody:  Phosphorylation of the Rb protein causes the inactivation of its growth inhibitory functions, causing the cell cycle to proceed into the late G1 phase. This Phospho-Rb antibody is excellent for Western blotting applications and was raised against a human Rb peptide phosphorylated at Ser780.

Phospho-Tau antibody: Tau is found to be hyperphosphorylated in paired helical filaments, the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease, making this proteins behaviour of great interest to neuroscientists. Phospho-Tau antibody is suitable for detection of hyperphosphorylated tau in Western blot of lysates and purified proteins.

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