Transcription Factors

Transcription factors are proteins that bind to specific DNA sequences in order to regulate transcription from DNA to mRNA by either activating or inhibiting RNA polymerase.Transcription factors form a large group of diverse DNA-binding proteins capable of activating gene regulation or repressing it. Some transcription factors, such as STAT and NFΚB, must be phosphorylated before they can bind DNA, while other transcription factors respond to ligand binding. Transcription factors are important regulators of cellular development making them a key area of research in the study of genetics, inherited disease, cancer, differentiation and development and the cell cycle in general.

Transcription factors can operate on their own or they may form homodimers and heterodimers with other factors. Each transcription factor may have multiple DNA targets of varying function, but which particular gene is transcribed can be spatially and temporally regulated by transcription co-factors, chromatin structure, noncoding RNAs, epigenetic marks, or localization of the transcription factor itself.

Fitzgerald Industries offers a wide range of high quality transcription factor antibodies ideal for use in research or diagnostic laboratories. Our range of transcription factor antibodies are available in monoclonal or polyclonal formats as well as conjugated to colorimetric and fluorimetric tags including biotin, fluorescein isothiocyanate (FITC), horseradish peroxidase (HRP), alkaline phosphate and others.

Some of our most popular transcription factor antibodies include:

Ubiquitin antibody: Ubiquitin is a transcription factor which plays a central role in cell-cycle regulation, DNA repair, cell growth, signalling and immune function. This monoclonal Ubiquitin antibody is affinity purified and is recommended for use in Immunoprecipitation, Western blotting and immunohistochemistry.

HDAC1 antibody: HDAC1 is an active component of transcriptional corepressor complexes which can be recruited to specific promoter regions via transcriptional factors, inhibiting gene expression. This polyclonal HDAC1 antibody is recommended for use in Western blot. In addition, we offer a monoclonal HDAC1 antibody which is excellent for immunofluoresence (as illustrated below) and Dot blots.

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-HDAC1 antibody.
Immunofluorescent staining of HeLa cells with HDAC1 antbody

 

SNRPD3 antibody:  SNRPD3 belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. This SNRPD3 antibody can be used in Dot-Blot as well as Immunocytochemistry applications.

TRIM32 antibody: TRIM32 is a member of the tripartite motif (TRIM) family and interacts with the activation domain of the HIV-1 Tat protein in the nucleus. This TRIM32 antibody is rated for use in Immunohistochemistry and Western blotting.

YY1 antibody: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. YY1 protein is responsible for repressing and activating a diverse number of promoters, and it has been implicated in histone modification. This mouse monoclonal YY1 antibody can be used in Westrn blotting, Dot blots, and in immunocytochemistry. 

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-YY1 antibody.
Immunofluorescent staining if HeLa cells using YY1 antibody

We have a huge range of transcription factor antibodies which can be used in Dot blots, Western blotting and in cell immunofluorescence.