Akt antibodies also known as Protein Kinase B antibodies or PKB antibodies are important for studying the serine/threonine protein kinase activity involved in the regulation of several cellular processes including metabolism, proliferation, cell survival, growth, angiogenesis and tumor formation. Akt achieves this through the serine and/or threonine phosphorylation of a range of downstream substrates. Akt is fully activated by phosphorylation at two key sites, Ser308 and Thr478.
Akt is made up of 3 family members. Akt1 antibodies (Akt antibodies) are used to examine regulation of apoptopic pathways through inhibition. Akt1 is also a key signalling protein in the cellular pathways in skeletal muscle and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt2 antibodies are used to study the Insulin signalling pathway. It is required to induce glucose transport through interaction with the insulin receptor pathway. Akt3 antibodies are used to define the less understood function of Akt3 which is believed to play a role in neural development.
With its widespread roles in cell biology, metabolism, neuroscience and cancer, Akt is of interest to researchers in many fields from medical diagnostics to the identification of new drug targets in the pharmaceutical and biotech industries.
Fitzgerald Industries offers an excellent selection of high quality Akt antibodies and proteins for research use. Our Akt antibodies are available in monoclonal and polyclonal formats from most common host species and rated for use in ELISA, Functional Assays, Immunohistochemistry, Western Blotting and more. Some of our most popular Akt antibodies include:
This rabbit polyclonal Akt antibody reacts with human, mouse, and rat Akt and was purified by affinity chromatography. It provides excellent results in both Western Blotting and Immunohistochemistry as illustrated below in human breast carcinoma tissue.
immunohistochemical analysis of paraffin-embedded human
breast carcinoma tissue using Akt antibody (left) or the same
antibody preincubated with with blocking peptide (right)
This polyclonal Akt2 antibody is highly purified and supplied in PBS buffer with preservative and has been validated for use both ELISA and Western Blot applications for detection of human Akt2.
This monoclonal Akt3 antibody (clone 9H3) provides excellent results in Immunofluorescence, Immunohistochemistry and Western Blot applications. It reacts with human and mouse Akt3.
Immunohistochemical analysis of AKT3 protein in paraffin embedded Carcinoma of Human lung tissue using AKT3 antibody
|Immunofluorescent staining of Hela cells transiently transfected with recombinant AKT3 protein using AKT3 antibody|