Estrogen receptor antibody
Estrogen receptor antibodies are used to study the hormone estrogen which is a small, carbon-rich hormones derived from cholesterol. Unlike larger hormones such as insulin which are bound by receptors on the cell surface, estrogens pass directly into cells interior and attach to receptors in the cells cytosol and/or nucleus. When estrogen enters the nucleus, it binds to the estrogen receptor forming a dimer. There are two different forms of the estrogen receptor, usually referred to as alpha and beta. Estrogen receptor alpha and beta show significant overall sequence similarities, although different ligands may differ in their affinity for alpha and beta isoforms of the estrogen receptor.
Once activated by estrogen, the Estrogen Receptor is a DNA-binding transcription factor, it is able to translocate into the nucleus and bind to DNA to regulate the activity of a wide variety of different genes governing development, reproduction, aging, metabolism and more. Dysfunction in Estrogen and the Estrogen Receptor has been closely linked to obesity, breast cancer, ovarian cancer, colon cancer, prostate cancer, and endometrial cancer.
Fitzgerald Industries offers a wide range of monoclonal and polyclonal Estrogen Receptor antibodies for research and further manufacture. Our high quality Estrogen Receptor antibodies are the ideal choice for use in immunoassays and other immunodiagnostic techniques.
Some of our most popular ER antibodies include:
Estrogen Receptor antibody - This protein A purified rat monoclonal Estrogen Receptor antibody is specific for human ER and is recommended for use in ELISA and Western blot applications.
Estrogen Receptor alpha antibody - This purified polyclonal Estrogen Receptor antibody is an excellent example of the alpha form of the ER antibody. It reacts with both human and mouse estrogen receptor alpha, and raised specifically using a peptide sequence around phosphorylation site of serine 118 (Q-L-S(p)-P-F) as the immunogen It is validated for use in Immunofluorescence, Immunohistochemistry and Western blot.
|IHC analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a (Phospho-Ser118) antibody (left) or the same antibody preincubated with with blocking peptide (right)||Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor alpha (Phospho-Ser118) antibody|
Estrogen Receptor beta antibody - This affinity purified rabbit polyclonal Estrogen Receptor beta antibody is speific for rat estrogen receptor, and cross reacts with human, mouse, non-human primate, rat, and sheep. It is useful in a large selection of immunodiagnostic techniques including Gel Shift, Immunocytochemistry, Immunofluorescence, IHC-F, IHC-P and Western Blot and is provided with full assay recommendations for use.