GAPDH antibodies are most commonly used as a loading control in Western blotting due to its constitutive expression. GAPDH or Glyceraldehyde 3-phosphate dehydrogenase is an important metabolic enzyme which catalyses the sixth step of glycolysis in the breakdown of glucose for energy during carbohydrate metabolism. GAPDH is present in high levels in practically all tissues. It is a multifunctional enzyme and as well as its main glycolytic function it is also implicated in several house-keeping functions in cell biology where it catalyzes the synthesis of 1,3-biphosphoglycerate, required for the synthesis of ATP. GAPDH is also involved in the early stages of apoptosis where it is translocated to the nucleus.
GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin, a protein that occurs naturally in nerve cells, but when mutated can cause Huntington's chorea. As with many proteins associated with cell proliferation, GAPDH has also been associated with several cancers and tumorigenesis.
Research is ongoing into the many diverse functions of GAPDH. Fitzgerald Industries offers a range of high quality monoclonal and polyclonal GAPDH antibodies, we also offer GAPDH antibodies conjugated to a range of useful tags including biotin, FITC, GST, HRP and GST tags. Our GAPDH antibodies are the ideal choice for use in procedures such as ELISA, Dot Blot, Immunocytochemistry, Immunofluorescence and more.
Some of our most popular GAPDH antibodies include:
GAPDH antibody - This mouse monoclonal GAPDH antibody is highly purified and can be used in ELISA, Immunocytochemistry and Western blotting. This is highly rated by our customers and reviewed on our website. It also has been referenced in literature over 15 times, making it one of the best GAPDH antibodies for research.
|Heart extracts were prepared from different animal species and 0.5 mg of homogenized cardiac tissue was loaded. Anti-mouse IgG (HRP) was used GAPDH immune complex visualization. Lane 1: isolated human GAPDH, 0.5 ug; Lane 2: human heart tissue extract; Lane 3: pig heart tissue extract; Lane 4: goat heart tissue extract; Lane 5: bovine heart tissue extract; Lane 6: dog heart tissue extract; Lane 7: mouse heart tissue extract; Lane 8: rat heart tissue extract; Lane 9: rabbit heart tissue extract; Lane 10: duck heart tissue extract|
GAPDH antibody - This affinity purified rabbit polyclonal GAPDH antibody offers excellent results in Immunofluorscent applications. This antibody reactes with drosophila, human, mouse, and rat GAPDH. Below shows confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using this GAPDH antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2000.
GAPDH antibody (FITC) - This polyclonal GAPDH antibody is one of the most popular of our range of conjugated GAPDH antibodies. It has been purified using Ammonium Sulfate precipitation. The GAPDH-FITC tag makes it an excellent choice for applications including ELISA, Immunohistochemistry and Western Blot.
Check out our full guide to GAPDH antibodies and proteins or see our matched GAPDH antibody pairs - capture GAPDH antibody and detection GAPDH antibody for use in ELISA, and which can also be used in immunocytochemistry and Western blotting.