Antigen 85A protein (His tag) (80R-2231)
Purified recombinant Mycobacterium tuberculosis Antigen 85A Protein (His tag)
|Synonyms||Antigen A-85 protein, Antigen A 85, Antigen 85 complex A protein, Mycolytransferase 85A protein, Antigen 85A, Antigen A-85, Fibronectin-binding protein A protein, Antigen A 85 protein|
Coomassie Blue stained SDS-PAGE of Antigen 85A protein (His tag) (80R-2231)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH|
|Expression System||Hi-5 cells|
|Purity||> 90% pure|
|Molecular Weight||32.8 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer, pH 8.0, containing, 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||The maintenance of the highly hydrophobic cell wall is central to the survival of Mycobacterium tuberculosis within its host environment. The antigen 85 proteins (85A, 85B, and 85C) of M. tuberculosis help maintain the integrity of the cell wall 1) by catalyzing the transfer of mycolic acids to the cell wall arabinogalactan and 2) through the synthesis of trehalose dimycolate (cord factor). Additionally, these secreted proteins allow for rapid invasion of alveolar macrophages via direct interactions between the host immune system and the invading bacillus.|
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