APEX1 protein ( T7 tag) (80R-1002)
Purified recombinant Human APEX1 protein ( T7 tag)
|Synonyms||APEX nuclease (multifunctional DNA repair enzyme) 1 protein, APE protein, APE1 protein, APEN protein, APX protein, HAP1 protein, REF-1 protein, REF1|
Coomassie Blue stained SDS-PAGE of APEX1 protein ( T7 tag) (80R-1002)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-318 amino acids: MASMTGGQQM GRGSMPKRGK KGAVAEDGDE LRTEPEAKKS KTAAKKNDKE AAGEGPALYE DPPDQKTSPS GKPATLKICS WNVDGLRAWI KKKGLDWVKE EAPDILCLQE TKCSENKLPA ELQELPGLSH QYWSAPSDKE GYSGVGLLSR QCPLKVSYGI GEEEHDQEGR VIVAEFDSFV LVTAYVPNAG RGLVRLEYRQ RWDEAFRKFL KGLASRKPLV LCGDLNVAHE EIDLRNPKGN KKNAGFTPQE RQGFGELLQA VPLADSFRHL YPNTPYAYTF WTYMMNARSK NVGWRLDYFL LSHSLLPALC DSKIRSKALG SDHCPITLYL AL|
|Purity||> 90% pure|
|Molecular Weight||36.9 kDa (332aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 2mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||APEX1, also known as REF1 or APE1 (Apurinic/apyrimidinic endonuclease), is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. APEX1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents. Recombinant human APEX1 protein, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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