APIP protein (His tag) (80R-2353)
Purified recombinant Human APIP Protein (His tag)
|Synonyms||CGI-29 protein, MMRP19 protein, APIP2 protein, Probable methylthioribulose-1-phosphate dehydratase protein, CGI29 protein, dJ179L10.2 protein|
Coomassie Blue stained SDS-PAGE of APIP protein (His tag) (80R-2353)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MGSHMSGCDA REGDCCSRRC GAQDKEHPRY LIPELCKQFY HLGWVTGTGG GISLKHGDEI YIAPSGVQKE RIQPEDMFVC DINEKDISGP SPSKKLKKSQ CTPLFMNAYT MRGAGAVIHT HSKAAVMATL LFPGREFKIT HQEMIKGIKK CTSGGYYRYD DMLVVPIIEN TPEEKDLKDR MAHAMNEYPD SCAVLVRRHG VYVWGETWEK AKTMCECYDY LFDIAVSMKK VGLDPSQLPV GENGIV|
|Purity||> 85% pure|
|Molecular Weight||29.7 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1-2 weeks). Aliquot and store at -20 deg C to -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||APIP catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). It has an anti-apoptotic function and prevents muscle ischemic damage. This protein inhibits the cytochrome c-dependent and APAF1-mediated cell death. Recombinant human APIP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
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