ApoA-IV antibody (10R-2160)
Mouse monoclonal ApoA-IV antibody
|Synonyms||Monoclonal ApoA-IV antibody, Anti-ApoA-IV antibody, Apolipoprotein A-Iv antibody, ApoA4 antibody, MGC142156 antibody, Apolipoprotein A lV antibody, MGC142154 antibody, Apo AIV antibody|
|Immunogen||ApoA-IV antibody was raised in Mouse using a purified recombinant fragment of APOA4 (aa21-396) expressed in E. coli as the immunogen.|
Western Blot analysis using ApoA-IV antibody (10R-2160)
Western Blot showing APOA4 antibody used against human serum (1), human plasma (2), HepG2 cell lysate (3) and SMMC-7721 cell lysate (4).
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||APOA4 is a 396-residue preprotein, which undergoes proteolytic processing to yield apo A-IV, a 376-reside mature O-linked glycoprotein. In most mammals, including humans, apo A-IV synthesis is confined to the intestine; however in mice and rats hepatic synthesis also occurs. Apo A-IV is secreted into circulation on the surface of newly synthesized chylomicron particles. Intestinal fat absorption dramatically increases the synthesis and secretion of apo A-IV, although its primary function in human lipid metabolism has not been established.|
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