ApoM antibody (10R-2093)

Mouse monoclonal ApoM antibody

Synonyms Monoclonal ApoM antibody, Anti-ApoM antibody, HSPC336 antibody, NG20 like protein antibody, G3a antibody, Apolipoprotein Mantibody, MGC22400 antibody, Protein G3a antibody, NG20 antibody
Cross Reactivity Human
Applications ELISA, IF, WB
Immunogen ApoM antibody was raised in Mouse using a purified recombinant fragment of ApoM expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using ApoM antibody (10R-2093) (ApoM antibody)

Immunofluorescence analysis of methanol-fixed L-02 (left) and Cos7 (right) cells using ApoM antibody showing cytoplasmic and membrane localization.

Specifications

Host Mouse
Clone 10C3G5
Isotype IgG2a
Form & Buffer Purified antibody supplied in PBS containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Apolipoproteins are protein components of plasma lipoproteins. APOM is a member of the lipocalin family of proteins. APOM is exclusively expressed in kidney tubular epithelial cells and liver hepatocytes. APOM is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions. The concentration of APOM in plasma strongly correlates with total cholesterol. Low concentrations of APOM in plasma are associated with diabetes. Recombinant human APOM protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Immunofluorescent staining using Western Blot analysis using ApoM antibody (10R-2093) (ApoM antibody) | Immunofluorescence analysis of methanol-fixed L-02 (left) and Cos7 (right) cells using ApoM antibody showing cytoplasmic and membrane localization.
  • Western Blot analysis using ApoM antibody (10R-2093) | Western Blot showing ApoM antibody used against GST-ApoM recombinant protein (1) and human serum (2).

Availability: In stock

Price: $375.00
Size: 100 ul
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