ARF1 antibody (10R-1783)
Mouse monoclonal ARF1 antibody
|Synonyms||Monoclonal ARF1 antibody, Anti-ARF1 antibody, ARF1, ADP ribosylation factor 1 antibody, ADP-ribosylation factor 1 antibody, ARF 1 antibody, ARF 1, ARF-1, ARF-1 antibody|
|Immunogen||ARF1 antibody was raised in mouse using recombinant human ARF1 (1-181aa) purified from E. coli as the immunogen.|
Western Blot analysis of ARF1 antibody (10R-1783)
The cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
|Source||Anti-human ARF1 antibody, clone 1B3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ARF1 protein.|
|Method of Purification||By protein-G affinity chromatography|
|Form & Buffer||Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide|
Usage & Assay Information
|Usage Recommendations||Each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000.|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family member is essential and ubiquitous in eukaryotes, being involved in vesicular transport and functioning as an activator of phospholipase D and cholera toxin. The functions of ARF proteins in membrane traffic and organelle integrity are intimately tied to its reversible association with membranes and specific interactions with membrane phospholipids. ARF1 is various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane.|
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