ARL11 protein (His tag) (80R-1886)
Purified recombinant ARL11 protein
|Synonyms||ARL 11, ARL 11 protein, ARL-11, ARLTS1 protein, ARL11, ARL-11 protein|
Coomassie Blue stained SDS-PAGE of ARL11 protein (His tag) (80R-1886)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-196 amino acids: MGSSHHHHHH SSGLVPRGSH MGSVNSRGHK AEAQVVMMGL DSAGKTTLLY KLKGHQLVET LPTVGFNVEP LKAPGHVSLT LWDVGGQAPL RASWKDYLEG TDILVYVLDS TDEARLPESA AELTEVLNDP NMAGVPFLVL ANKQEAPDAL PLLKIRNRLS LERFQDHCWE LRGCSALTGE GLPEALQSLW SLLKSRSCMC LQARAHGAER GDSKRS|
|Purity||> 90% pure|
|Molecular Weight||23.6kDa (216aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 5mM DTT, 30% glycerol, 100mM NaCl and 1mM EDTA.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ARL11(ADP-ribosylation factor-like protein 11), is a member of the ARF family of the Ras superfamily of small GTPases that are known to be involved in multiple regulatory pathways altered in human carcinogenesis. ARFs are highly conserved guanine nucleotide binding proteins that enhance the ADP-ribosyltransferase activity of choleratoxin. ARFs are important in eukaryotic vesicular trafficking pathways and they play an essential role in the activation of phospholipase D (PC-PLD). ARL11 is thought to function as a tumor suppressor that may play a role in the regulation of apoptosis. Recombinant human ARL11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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