ARNTL2 antibody (10R-1237)

Mouse monoclonal ARNTL2 antibody

Synonyms Monoclonal ARNTL2 antibody, Anti-ARNTL2 antibody, MOP9 antibody, ARNTL 2 antibody, ARNTL 2, ARNTL-2 antibody, CLIF antibody, ARNTL-2, PASD9 antibody, BMAL2 antibody, Aryl hydrocarbon receptor nuclear translocator-like 2 antibody, ARNTL2
Specificity Human ARNTL2
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, FC, ICC, WB
Immunogen ARNTL2 antibody was raised in mouse using recombinant Aryl Hydrocarbon Receptor Nuclear Translocator-Like 2

Images

Flow Cytometric assay using ARNTL2 antibody (10R-1237)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ARNTL2 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone ARNTH6B5
Isotype IgG1
Method of Purification ARNTL2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer ARNTL2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance BMAL2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription. Also, in umbilical vein endothelial cells, activates SERPINE1 through E-box sites. This transactivation is inhibited by PER2 and CRY1.

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Images

  • Flow Cytometric assay using ARNTL2 antibody (10R-1237) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ARNTL2 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using ARNTL2 antibody (10R-1237)
  • Immunofluorescent staining using ARNTL2 antibody (10R-1237) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ARNTL2 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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