ASH2L antibody (10R-1325)

Mouse monoclonal ASH2L antibody

Synonyms Monoclonal ASH2L antibody, Anti-ASH2L antibody, ASHL-2 antibody, ASH2 antibody, ASHL 2 antibody, ASH2L1 antibody, Ash2 histone methyltransferase complex subunit antibody, ASH2L2 antibody, ASH2L, ASHL 2, ASHL-2
Specificity Human ASH2L
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, FC, ICC, WB
Immunogen ASH2L antibody was raised in mouse using recombinant Human Ash2 (Absent, Small, Or Homeotic)-Like (Drosophila) (Ash2L)

Images

Flow Cytometric assay using ASH2L antibody (10R-1325)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ASH2L antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone 2046D2a
Isotype IgG1
Method of Purification ASH2L antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer ASH2L antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. May function as a transcriptional regulator. May play a role in hematopoiesis.

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Images

  • Flow Cytometric assay using ASH2L antibody (10R-1325) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ASH2L antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using ASH2L antibody (10R-1325)
  • Immunofluorescent staining using ASH2L antibody (10R-1325) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ASH2L antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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