ATG4B protein (His tag) (80R-2175)
Purified recombinant Human ATG4B protein (His tag)
|Synonyms||Cysteine protease ATG4B protein, Autophagin 1 protein, ATGB 4 protein, AUTL1 protein, Autophagin-1 protein, ATGB 4, ATGB-4, ATGB-4 protein, ATG4B|
Coomassie Blue stained SDS-PAGE of ATG4B protein (His tag) (80R-2175)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL WFTYRKNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ RKRQPDSYFS VLNAFIDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRTSVPCA GATAFPADSD RHCNGFPAGA EVTNRPSPWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVEPTD GCFIPDESFH CQHPPCRMSI AELDPSIAVG FFCKTEDDFN DWCQQVKKLS LLGGALPMFE LVEQQPSHLA CPDVLNLSLD SSDVERLERF FDSEDEDFEI LSLLEHHHHH H|
|Purity||> 90% pure|
|Molecular Weight||45.4 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ATG4B is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.|
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