ATG5 protein (His tag) (80R-1661)
Purified recombinant Human ATG5 protein
Overview
Overview
| Synonyms | hAPG5, ATG5, APG5 protein, APG5-LIKE protein, ATG 5 protein, ASP protein, ATG-5 protein, ATG 5, Autophagy protein 5 protein, APG5L protein, ATG-5 |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of ATG5 protein (His tag) (80R-1661)
Figure annotation denotes ug of protein loaded and % gel used.
ATG5 protein (His tag)
Coomassie Blue stained SDS-PAGE of ATG5 protein (His tag) (80R-1661)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-275 amino acids: MGSSHHHHHH SSGLVPRGSH MTDDKDVLRD VWFGRIPTCF TLYQDEITER EAEPYYLLLPRVSYLTLVTD KVKKHFQKVM RQEDISEIWF EYEGTPLKWH YPIGLLFDLL ASSSALPWNITVHFKSFPEK DLLHCPSKDA IEAHFMSCMK EADALKHKSQ VINEMQKKDH KQLWMGLQND RFDQFWAINR KLMEYPAEEN GFRYIPFRIY QTTTERPFIQ KLFRPVAADG QLHTLGDLLKEVCPSAIDPE DGEKKNQVMI HGIEPMLETP LQWLSEHLSY PDNFLHISII PQPTD |
|---|---|
| Expression System | E.coli |
| Purity | > 85% pure |
| Molecular Weight | 34.6 kDa (295aa), confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 40% glycerol and 0.2M NaCl. |
| Concentration | 0.25 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Autophagy protein 5, also known as ATG5, belongs to the autophagy-related (ATG) genes family. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. ATG5 protein is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Recombinant human ATG5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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