ATGL antibody (10R-1132)

Mouse monoclonal ATGL antibody

Synonyms Monoclonal ATGL antibody, Anti-ATGL antibody, calcineurin-dependent 1 antibody, cytoplasmic antibody, patatin-like phospholipase domain containing 2 antibody, NFATc antibody, nuclear factor of activated T-cells antibody, NFAT2 antibody, NF-ATC antibody
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, IHC, WB
Immunogen ATGL antibody was raised in mouse using recombinant human ATGL (30-504aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of ATGL antibody (10R-1132)

The extracts of mouse kidney (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human ATGL (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT2G2
Isotype IgG2b kappa
Source Anti-human ATGL antibody, clone AT2G2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ATGL protein.
Method of Purification ATGL antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.

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Images

  • Western Blot analysis of ATGL antibody (10R-1132) | The extracts of mouse kidney (20 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human ATGL (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $295.00
Size: 100 ul
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