ATP5D protein (His tag) (80R-1858)

Purified recombinant ATP5D protein

Synonyms ATP synthase subunit delta protein, ATPD 5, ATP5D, F-ATPase delta subunit protein, ATPD-5, ATPD 5 protein, ATPD-5 protein, mitochondrial protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of ATP5D protein (His tag) (80R-1858)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 23-168 amino acids: MGSSHHHHHH SSGLVPRGSH MAEAAAAPAA ASGPNQMSFT FASPTQVFFN GANVRQVDVP TLTGAFGILA HVPTLQVLR PGLVVVHAED GTTSKYFVSS GSIAVNADSS VQLLAEEAVT LDMLDLGAAK ANLEKAQAEL VGTADEATRA EIQIRIEANE ALVKALE
Expression System E.coli
Purity > 95% pure
Molecular Weight 17.3 kDa (167aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol and 0.1M NaCl.
Concentration 0.25 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance ATP5D, also known as F-ATPase delta subunit, catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This protein is the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. Recombinant human ATP5D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of ATP5D protein (His tag) (80R-1858) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 10 ug
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