Axin1 antibody (10R-1186)

Mouse monoclonal Axin1 antibody

Synonyms Monoclonal Axin1 antibody, Anti-Axin1 antibody, AXIN antibody, Axin -1, Axis inhibition protein 1 antibody, Axin 1, Axin1, hAxin antibody, Axin -1 antibody, Axin 1 antibody, Axin-1 antibody, axin 1 antibody, MGC52315 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen Axin1 antibody was raised in mouse using recombinant human Axin1 (546-752aa) purified from E. coli as the immunogen.

Images

Immunofluorescent staining using Axin1 antibody (10R-1186)

Immunofluorescenitrocellulosee of stained human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) for monoclonal anti-human Axin1a antibody (1:500).

Specifications

Host Mouse
Clone AT1A4
Isotype IgG2b kappa
Source Anti-human Axin1 antibody, clone AT1A4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Axin1 protein.
Method of Purification Axin1 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-:500, WB: 1:250-1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Axin was originally identified as the product of the mouse gene called ?fused? or fu (renamed Axin), and has since been shown to play a crucial role in controlling axis formation during embryonic development. Axin1 is characterized to be a tumor suppressor and numerous gene mutations have been identified throughout Axin1 from a number of different cancers. Axin can identify in a mouse mutant strain, created by random integration of a transgene. Also, Axin1 plays an important role in the formation of the embryonic neural axis. Recently, it was reported that Axin is found in mitotic spindles and centrosomes.

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Images

  • Immunofluorescent staining using Axin1 antibody (10R-1186) | Immunofluorescenitrocellulosee of stained human HeLa cells was done with Hoechst 33342 (Blue) for chromatin staining and Texas Red (Red) for monoclonal anti-human Axin1a antibody (1:500).
  • Western Blot analysis of Axin1 antibody (10R-1186) | Axin1 has 44% homology with Axin2 (Conductin) and its funitrocellulosetion is similar, but they have different expression patterns. Axin1 is widely expressed and detected at a very low level, whereas Axin2 is differentially expressed in various cells and tissue (stomach canitrocelluloseer or colorectal canitrocelluloseer). Also, it is reported that Axin2 was highly over-expressed in AGS and SW480 cell lines.  Immunoprecipitation (IP) and western blot experiments were performed to evaluate Axin1 antibody’s specificity. IP sample and cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-mouse Axin1 (1:500). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $265.00
Size: 100 ul
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