AXIN2 antibody (20R-3003)

Rabbit polyclonal AXIN2 antibody

Synonyms Polyclonal AXIN2 antibody, Anti-AXIN2 antibody, Axis inhibition protein 2 antibody, Axin-like protein antibody, Axil antibody, Axis inhibition protein 2 antibody, Conductin antibody
Applications IHC-P, WB
Immunogen AXIN2 antibody was raised in Rabbit using KLH-conjugated linear peptide corresponding to human Axin2 as the immunogen

Images

Immunohistochemical staining using AXIN2 antibody (20R-3003)

Paraffin-embedded colorectal cancer (Fig. 1) and multiform glioblastoma (Fig. 2 & 3) tissues were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Anti-Axin-2. Staining pattern appears to be restricted to malignant cells.

Specifications

Host Rabbit
Form & Buffer Suppliedin buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations WB: 0.5 ug/m, IHC-P: 1:500

Storage & Safety

Storage Stable for 1 year at 2-8 deg C from date of receipt

General Information

Biological Significance Axis inhibition protein 2 (AXIN2) has been linked to selective tooth angenesis. It has been found in human thymus, prostate, testis, small intestine, ovarian tissue and in the colon, at a lower expression rate. Axin-2 is involved during skull morphogenesis and missing Axin-2 can cause abnormal skull morphology. Variation in Axin-2 has been associated with accelerated chondrocyte proliferation.

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Images

  • Immunohistochemical staining using AXIN2 antibody (20R-3003) | Paraffin-embedded colorectal cancer (Fig. 1) and multiform glioblastoma (Fig. 2 & 3) tissues were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Anti-Axin-2. Staining pattern appears to be restricted to malignant cells.
  • Western Blot analysis using AXIN2 antibody (20R-3003) | SW620 cell lysate was probed with AXIN2 antibody (20R-3003) (0.5 ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.Arrow indicates AXIN2 (~85 kDa)

Availability: In stock

Price: $295.00
Size: 100 ul
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