BCL2 antibody (10R-2218)

Mouse monoclonal BCL2 antibody for detection of BCL2 in the research laboratory

Synonyms Monoclonal BCL2 antibody, Anti-BCL2 antibody, BCL-2 antibody, BCL 2 antibody, B-cell lymphoma protein 2 alpha antibody, B-cell lymphoma protein 2 alpha Apoptosis regulator Bcl 2 antibody, Apoptosis regulator Bcl2 antibody, AW986256 antibody, B cell CLL/lymphoma 2 antibody, B cell leukemia/lymphoma 2 antibody, Bcl-2 antibody, Bcl2 antibody, C430015F12Rik antibody, D630044D05Rik antibody, D830018M01Rik antibody, Leukemia/lymphoma antibody, B-cell antibody, Oncogene B-cell leukemia 2 antibody, BCL2, BCL 2, BCL-2, BCL 2 antibody, BCL-2 antibody
Cross Reactivity Human, Mouse
Applications ELISA, IF, IHC
Immunogen BCL2 antibody was raised in Mouse using synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH, as the immunogen.

Images

Flow Cytometric assay using (BCL2 antibody)

FC analysis of 3T3L1 cells using BCL-2 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 8E12
Isotype IgG1
Form & Buffer BCL2 antibody is supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance BCL2 is an anti-apoptotic protein located primarily in the outer mitochondrial membrane. It blocks the apoptotic death of some cells such as lymphocytes. BCL2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apoptosis.

References
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Images

  • Flow Cytometric assay using  (BCL2 antibody) | FC analysis of 3T3L1 cells using BCL-2 antibody (green) and negative control (purple).
  • Immunohistochemical staining using BCL2 antibody (10R-2218) | Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human brain tissues (right) using BCL-2 antibody with DAB staining.
  • Immunofluorescent staining using  (BCL2 antibody) | Immunofluorescence analysis of NIH/3T3 cells using BCL-2 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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