BDH2 protein (His tag) (80R-1640)
Purified recombinant Human BDH2 protein
|Synonyms||UCPA-OR protein, BDH-2 protein, SDR15C1 protein, PRO20933 protein, BDH-2, BDH 2, BDH2, FLJ13261 protein, DHRS6 protein, BDH 2 protein, UNQ6308, EFA6R protein, 3-hydroxybutyrate dehydrogenase type 2 protein|
Coomassie Blue stained SDS-PAGE of BDH2 protein (His tag) (80R-1640)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-245 amino acids: MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAN EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL|
|Purity||> 95% pure|
|Molecular Weight||28.8 kDa (265aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||BDH2, also known as DHRS6, belongs to the short-chain dehydrogenases/reductases (SDR) family. This protein may play an important role in the peripheral utilization of 3-hydroxybutyrate. The cytoplasmic localization with its high ratio of oxidized NAD+, the NAD+ dependence and the kinetic parameters of BDH2 make it suitable to convert high levels of circulating 3-hydroxybutyrate into acetoacetate. Recombinant human BDH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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