beta Actin antibody (10R-2013)

Mouse monoclonal beta Actin antibody

Synonyms Monoclonal beta Actin antibody, Anti-beta Actin antibody, PS1TP5-binding protein 1 antibody, ACTB antibody, PS1TP5BP1 antibody, beta-actin antibody, b-actin antibody
Cross Reactivity Human, Mouse, Hamster, Rat, Monkey
Applications ELISA, FC, IF, WB
Immunogen beta Actin antibody was raised in Mouse using synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using Beta Actin antibody (10R-2013) (Beta Actin antibody)

FC analysis of MCF-7 cells using beta Actin antibody (right) and negative control (left).

Specifications

Host Mouse
Clone 8H10D10
Isotype IgG2b
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, FC: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Beta-actin is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

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Images

  • Flow Cytometric assay using Western Blot analysis using Beta Actin antibody (10R-2013) (Beta Actin antibody) | FC analysis of MCF-7 cells using beta Actin antibody (right) and negative control (left).
  • Western Blot analysis using Beta Actin antibody (10R-2013) | Western Blot showing beta-Actin antibody used against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
  • Immunofluorescent staining using Western Blot analysis using Beta Actin antibody (10R-2013) (Beta Actin antibody) | Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin antibody (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.

Availability: In stock

Price: $345.00
Size: 100 ul
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