beta Catenin ELISA kit (55R-IB09614)

ELISA kit for the detection of beta Catenin in the research laboratory

Synonyms beta Catenin ELISA kit, CTNNB ELISA kit,
Applications ELISA
Assay Information This betacatenin ELISA kit is a complete kit for the determination of betacatenin in cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to betacatenin immobilized on a microtiter plate to bind the betacatenin in the standards or sample. A recombinant human betacatenin Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a polyclonal antibody to betacatenin is added. This antibody binds to the betacatenin captured on the plate. After a short incubation the excess antibody is washed out and Goat antiRabbit IgG conjugated to Horseradish peroxidase is added, which binds to the polyclonal betacatenin antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of betacatenin in either standards or samples.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 2-8 deg C

General Information

Biological Significance beta Catenin is a 781 amino acid adherens junction protein that plays an important role in two unrelated cellular functions. Adherens junctions are critical for establishment and maintenance of epithelial layers, such as those found lining the surface of organs. Involved in both cell-cell adhesion and in the wnt/wg signaling pathway, beta Catenin can be found in the cytoplasm or the nucleus. In the cytoplasm, beta Catenin is unstabilized by phosphorylation and association with E-cadherin. When beta Catenin translocates to the nucleus, it has comparatively low levels of phosphorylation. Wnt/wg signaling results in beta Catenin accumulation and transcriptional activation of specific target genes during development. Deregulation of beta Catenin signaling is an important event in the evolution a number of malignancies, including colon cancer, melanoma, ovarian cancer, and prostate cancer. The homology between human, mouse and rat betacatenin is 99.9%.

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Availability: In stock

Price: $805.00
Size: 96 wells
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