BFIT protein (His tag) (80R-3569)

Purified recombinant BFIT protein (His tag)

Synonyms Acyl-CoA thioester hydrolase 11 protein, Acyl-CoA thioesterase 11 protein, Acyl-coenzyme A thioesterase 11 protein, Adipose-associated thioesterase protein, Brown fat-inducible thioesterase protein, KIAA0707 protein, THEA protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie blue stained SDS-PAGE of BFIT protein (His tag) (80R-3569)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 19-250 aa: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSNRTS RKSALRAGND SAMADGEGYR NPTEVQMSQL VLPCHTNQRG ELSVGQLLKW IDTTACLSAE RHAGCPCVTA SMDDIYFEHT ISVGQVVNIK AKVNRAFNSS MEVGIQVASE DLCSEKQWNV CKALATFVAR REITKVKLKQ ITPRTEEEKM EHSVAAERRR MRLVYADTIK DLLANCAIQG DLESRDCSRM VPAEKTRVES VELVLPPHAN HQGNTFGGQI MAWMENVA
Expression System E.coli
Purity > 90% pure
Method of Purification BFIT protein was purified by using conventional chromatography techniques
Molecular Weight 29.9 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea,10% glycerol
Concentration 1 mg/ml

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance BFIT is a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. The protein has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates.

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Images

  • Coomassie blue stained SDS-PAGE of BFIT protein (His tag) (80R-3569) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $275.00
Size: 100 ug
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