Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are an important element in reducing signal noise in a blotting procedure as well as to reduce non-specific binding of detection reagents to the membranes and microplates in antibody-based detection procedures. Casein buffer is also commonly used to block background binding of antibodies in ELISA assays as well as for use in Immunohistochemistry and nucleic acid detection methods.
Fitzgerald Industries supplies high quality, stable, concentrated Caesin Buffer ideally suited to most blotting procedures as well as biotin free Caesin Buffer. We also supply Caesin-based Stabilizing Buffer in a ready to use format for use as a drying buffer.
Why not visit our homepage today to see our full range of diluents and other buffers as well as our huge selection of monoclonal and polyclonal antibodies perfectly suited to Immunoblotting procedures.