Immunohistochemistry protocol

Immunohistochemistry (IHC) is a powerful process used for detecting antigens in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. As well as being widely used in biochemical research for detecting the expression and localization of target antigens and other biomarkers, immunohistochemical staining is also commonly used in the detection of abnormal cells such as those found in cancerous tumours. In IHC, target antigens are evaluated using specific antibodies directly conjugated with enzyme or fluorophore-conjugated antibodies, or indirectly using similarly labeled, secondary antibodies and reagents. A successful immunohistochemistry procedure requires the highest quality primary and secondary antibodies.

Fitzgerald Industries offers a wide range of high quality products suitable for immunohistochemistry applications, we supply thousands of primary antibodies, antigens, conjugated antibodies, native and recombinant proteins and reagents all validated for use in immunohistochemistry experiments procedures.


Top IHC antibodies


Our current top primary antibody targets are:


Influenza A antibody

This influenza A antibody is highly specific for Influenza A protein and does not cross react with Influenza B.

Troponin I antibody (Cardiac)

Troponin I is a widely studied cardiac marker and this cTI antibody is excellent for use in IHC of cardiac tissues.

Insulin+Proinsulin antibody

This antibody reacts with both proinsulin and insulin.

CA 125 antibody

This Cancer Antigen 125 antibody is one of our best IHC antibodies for detection of ovarian cancer biomarker CA 125 (also known as MUC16).

Neisseria gonorrhoeae antibody

 A highly sensitive N. gonorrhoeae antibody for use in immunohistochemistry.


See our Immunohistochemistry Protocol for instructions on carrying out your own IHC procedure.


Why not go to our homepage and search for antibodies for use in IHC.