Biotin reagent (Alk Phos) (65C-CE0201)

Alkaline Phosphatase conjugated biotin labelling reagent

Protein Type Synthetic
Applications ELISA, IHC, WB

Specifications

Method of Purification Biotin reagent (Alk Phos) was purified by ion exchange chromatography followed by dialysis.
Tag/Conjugate Alkaline Phosphatase
Form & Buffer 0.05M TRIS-HCl, pH 8.0, with 0.15M NaCl, 0.001M MgCl, 0.0001M ZnCl, 50% glycerol, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500

Storage & Safety

Storage Store at 4 deg C. DO NOT FREEZE.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Biotin is a water-soluble B-complex vitamin (vitamin B7) that is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis.

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Availability: In stock

Price: $155.00
Size: 1 mg
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