BNP protein (His tag) (80R-1069)
Purified recombinant Human BNP protein
|Synonyms||NPPB protein, Brain natriuretic peptide (type B natriuretic peptide) Gamma brain natriuretic peptide protein, GC-B protein, BNP protein, Brain natriuretic peptide (type B natriuretic peptide) protein, Natriuretic peptide precursor B protein, Natriuretic peptides B protein,|
Coomassie Blue stained SDS-PAGE of BNP protein (His tag) (80R-1069)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||27-134 amino acids: MGSSHHHHHH SSGLVPRGSH MHPLGSPGSA SDLETSGLQE QRNHLQGKLS ELQVEQTSLE PLQESPRPTG VWKSREVATE GIRGHRKMVL YTLRAPRSPK MVQGSGCFGR KMDRISSSSG LGCKVLRRH|
|Purity||> 90% pure|
|Molecular Weight||14 kDa (129 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in PBS, pH 7.4, containing 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||BNP (Brain natriuretic peptide) is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. BNP is a 32 amino acid polypeptide and is co-secreted along with a 76 amino acid N-terminal fragment which is biologically inactive. This protein has biological actions including natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Recombinant human BNP, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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