BPGM protein (His tag) (80R-1292)

Purified recombinant Human BPGM protein

Synonyms 2 protein, Bisphosphoglycerate mutase 2 protein, C86192., Ab2 098 protein, BPG-dependent PGAM protein, 3-bisphosphoglycerate synthase protein, erythrocyte protein, 3-bisphosphoglycerate mutase protein, 2 protein, 3-bisphosphoglycerate mutase protein, AI323730 AL022789 protein, BPG dependent PGAM protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of BPGM protein (His tag) (80R-1292)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-259 amino acids: MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF EFDLVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNER HYGALIGLNR EQMALNHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD QLPRSESLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED QGKVKQAKKL EHHHHHH
Expression System E.coli
Purity > 95% pure
Molecular Weight 31 kDa (267aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2,3-BPG concentration. It is responsible for the catalytic synthesis of 2,3-Bisphosphoglycerate (2,3-BPG) from 1,3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active. Recombinant human BPGM, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

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Images

  • Coomassie Blue stained SDS-PAGE of BPGM protein (His tag) (80R-1292) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $220.00
Size: 100 ug
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