BRAF antibody (10R-2099)

Mouse monoclonal BRAF antibody

Synonyms Monoclonal BRAF antibody, Anti-BRAF antibody, V-raf murine sarcoma viral oncogene homolog B1 antibody, BRAF1 antibody, B-RAF1 antibody, RAFB1 antibody, B RAF1 antibody
Cross Reactivity Human
Applications ELISA, IHC, WB
Immunogen BRAF antibody was raised in Mouse using a purified recombinant fragment of BRAF expressed in E. coli as the immunogen.

Images

Immunohistochemical staining using BRAF antibody (10R-2099)

Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF antibody.

Specifications

Host Mouse
Clone 1H12F1
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance BRAF is one of the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylation sites. B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras.

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Images

  • Immunohistochemical staining using BRAF antibody (10R-2099) | Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF antibody.
  • Western Blot analysis using BRAF antibody (10R-2099) | Western Blot showing BRAF antibody used against truncated recombinant Braf (1) and A431 cell lysate (2).
  • Western Blot analysis using BRAF antibody (20108) | Western Blot showing using BRAF antibody used against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).
  • Immunohistochemical staining using BRAF antibody (10R-2099) | Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue (right) showing cytoplasmic localization using BRAF antibody with DAB staining.

Availability: In stock

Price: $425.00
Size: 100 ul
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