BRD3 antibody (10R-1296)

Mouse monoclonal BRD3 antibody

Synonyms Monoclonal BRD3 antibody, Anti-BRD3 antibody, ORFX antibody, BRD 3, KIAA0043 antibody, BRD 3 antibody, BRD3, BRD-3, RING3L antibody, Bromodomain containing protein 3 antibody, BRD-3 antibody
Specificity Human BRD3
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, FC, ICC, IP, WB
Immunogen BRD3 antibody was raised in mouse using recombinant Human Bromodomain Containing 3 (Brd3)

Images

Flow Cytometric assay using BRD3 antibody (10R-1296)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BRD3 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone 2088C3a
Isotype IgG1
Method of Purification BRD3 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer BRD3 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.

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Images

  • Flow Cytometric assay using BRD3 antibody (10R-1296) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BRD3 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using BRD3 antibody (10R-1296)
  • Immunofluorescent staining using BRD3 antibody (10R-1296) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-BRD3 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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