BRPF1 antibody (10R-1297)

Mouse monoclonal BRPF1 antibody

Synonyms Monoclonal BRPF1 antibody, Anti-BRPF1 antibody, Bromodomain and PHD finger containing protein antibody, BRPF 1 antibody, BRPF-1 antibody, BRPF-1, BRPF 1, BR140 antibody, BRPF1
Specificity Recognises full lenght Human BRPF1 protein
Cross Reactivity Human, Mouse. Other species not studied
Applications Dot Blot, FC, IP, WB
Immunogen BRPF1 antibody was raised in mouse using recombinant Human Bromodomain And Phd Finger Containing, 1 (Brpf1)



Host Mouse
Clone 2093C5a
Isotype IgG1
Method of Purification BRPF1 antibody was purified using protein G column chromatography from culture supernatant of hybridomacultured in a medium containing bovine IgG-depleted(approximately 95%) fetal bovine serum.
Form & Buffer BRPF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, FC: 0.5-2 ug/sample

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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  • Western Blot analysis using BRPF1 antibody (10R-1297) | Detection of BRPF1 by Western blot. Samples Used: Whole cell lysate from human HeLa (50 ug) and rat NIH3T3 (50 ug) cells.
  • Western Blot analysis of immunized recombinant protein using BRPF1 antibody (10R-1297)
  • Flow Cytometric assay using BRPF1 antibody (10R-1297) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BRPF1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Availability: In stock

Price: $190.00
Size: 50 ug
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