BTC protein (His tag) (80R-2003)

Recombinant human BTC protein (His tag)

Synonyms Betacellulin protein, probetacellulin precursor protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of BTC protein (His tag) (80R-2003)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 32-111 amino acids: MGSSHHHHHH SSGLVPRGSH MDGNSTRSPE TNGLLCGDPE ENCAATTTQS KRKGHFSRCP KQYKHYCIKG RCRFVVAEQT PSCVCDEGYI GARCERVDLF Y
Expression System E.coli
Purity > 90% pure
Molecular Weight 11.3kD (101aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 20% glycerol, 200mM NaCl
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations BTC protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance BTC (Betacellulin) is a member of the EGF family of growth factors and contains 1 EGF-like domain. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. The effects of BTC are probably mediated by the EGF receptor and other related receptors. Recombinant human BTC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of BTC protein (His tag) (80R-2003) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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