BTF3 antibody (10R-1391)

Mouse monoclonal BTF3 antibody

Synonyms Monoclonal BTF3 antibody, Anti-BTF3 antibody, BTF3, BTF3b antibody, BTF 3 antibody, BTF-3, Basic transcription factor 3 antibody, BETA-NAC antibody, BTF-3 antibody, BTF 3, BTF3a antibody
Specificity Human BTF3
Cross Reactivity Human. Other species not studied
Applications Dot Blot, FC, ICC, WB
Immunogen BTF3 antibody was raised in mouse using recombinant Human Basic Transcription Factor 3

Images

Flow Cytometric assay using BTF3 antibody (10R-1391)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BTF3 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone 628C5a
Isotype IgG2b
Method of Purification BTF3 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer BTF3 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.

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Images

  • Flow Cytometric assay using BTF3 antibody (10R-1391) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BTF3 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using BTF3 antibody (10R-1391)
  • Immunofluorescent staining using BTF3 antibody (10R-1391) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-BTF3 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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