BUB1 antibody (10R-1316)

Mouse monoclonal BUB1 antibody

Synonyms Monoclonal BUB1 antibody, Anti-BUB1 antibody, BUB-1 antibody, BUB1, BUB1L antibody, BUB-1, hBUB1 antibody, BUB 1, BUB 1 antibody, BUB1A antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody
Specificity Human BUB1
Cross Reactivity Human. Other species not studied
Applications Dot Blot
Immunogen BUB1 antibody was raised in mouse using recombinant Human Bub1 Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (Bub1)

Images

Western Blot analysis of immunized recombinant protein using BUB1 antibody (10R-1316)

Specifications

Host Mouse
Clone 2096C1a
Isotype IgG1
Method of Purification BUB1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer BUB1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations To be optimized by the end user.

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance BUB1 (budding uninhibited by benzimidazoles 1) is an enzyme that in humans is encoded by the BUB1 gene. Bub1 is a serine/threonine protein kinase first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans.

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Images

  • Western Blot analysis of immunized recombinant protein using BUB1 antibody (10R-1316)

Availability: In stock

Price: $190.00
Size: 50 ug
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