BUB1B antibody (10R-1666)

Mouse monoclonal BUB1B antibody

Synonyms Monoclonal BUB1B antibody, Anti-BUB1B antibody, Budding uninhibited by benzimidazoles 1 homolog beta antibody, MAD3L antibody, BUBR1 antibody, BUBB-1 antibody, hBUBR1 antibody, BUBB-1, BUBB 1, Bub1A antibody, BUB1B, BUBB 1 antibody, BUB1beta antibody, SSK1 antibody
Specificity Human BUB1B
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen BUB1B antibody was raised in mouse using recombinant Human Bub1 Budding Uninhibited By Benzimidazoles 1 Homolog Beta (Yeast) (Bub1B)

Images

Immunofluorescent staining using BUB1B antibody (10R-1666)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-BUB1B antibody.

Specifications

Host Mouse
Clone 2097C2a
Isotype IgG2a
Method of Purification BUB1B antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer BUB1B antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphasepromoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

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Images

  • Immunofluorescent staining using BUB1B antibody (10R-1666) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-BUB1B antibody.
  • Western Blot analysis of immunized recombinant protein using BUB1B antibody (10R-1666)

Availability: In stock

Price: $190.00
Size: 50 ug
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