BUD31 protein (His tag) (80R-2840)
Purified recombinant BUD31 protein (His tag)
|Synonyms||BUD31, BUD 31, BUD-31, BUD31, BUD 31 protein, BUD-31 protein, BUD31 homolog protein, BUD31 homolog (S. cerevisiae) protein, Cwc14 protein, EDG-2 protein, EDG2 protein, fSAP17 protein, G10 protein, YCR063W protein|
SDS-PAGE analysis of BUD31 protein (His tag) (80R-2840)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-144 aa: MGSSHHHHHH SSGLVPRGSH MGSMPKVKRS RKAPPDGWEL IEPTLDELDQ KMREAETEPH EGKRKVESLW PIFRIHHQKT RYIFDLFYKR KAISRELYEY CIKEGYADKN LIAKWKKQGY ENLCCLRCIQ TRDTNFGTNC ICRVPKSKLE VGRIIECTHC GCRGCSG|
|Purity||> 85% pure|
|Molecular Weight||19.4 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||BUD31 is a nuclear protein that belongs to the BUD31 (G10) family. It contains a putative nuclear translocation sequence, an N-terminal acidic domain and a cysteine-rich C-terminal domain containing a putative Zinc-finger structure. The structure of BUD31 suggests that it may be a nuclear regulator of transcription. Recombinant human BUD31 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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