BXDC1 antibody (10R-1299)

Mouse monoclonal BXDC1 antibody

Synonyms Monoclonal BXDC1 antibody, Anti-BXDC1 antibody, BXDC-1, BXDC 1, RPF2 antibody, BXDC1, bA397G5.4 antibody, Ribosome production factor 2 homolog antibody, FLJ21087 antibody, BXDC 1 antibody, BXDC-1 antibody
Specificity Human BXDC1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen BXDC1 antibody was raised in mouse using recombinant Human Brix Domain Containing 1 (Bxdc1)

Images

Immunofluorescent staining using BXDC1 antibody (10R-1299)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-BXDC1 antibody.

Specifications

Host Mouse
Clone 2099C7a
Isotype IgG1
Method of Purification BXDC1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer BXDC1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance BXDC1 (Brix domain-containing protein 1) is a 306 amino acid protein encoded by the human gene BXDC1. BXDC1 is a nuclear protein that contains one Brix domain. Brix domain-containing proteins represent a family of proteins involved in the biogenesis of large ribosomal subunits. The Brix domain is a region with homology to the yeast protein Pitx1 (ribosome biogenesis protein BRX1). Pitx1 is part of a complex that includes RPF1, RPF2 and SSF1 or SSF2. This complex is required for the biogenesis of the 60S ribosomal subunit.

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Images

  • Immunofluorescent staining using BXDC1 antibody (10R-1299) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-BXDC1 antibody.
  • Western Blot analysis of immunized recombinant protein using BXDC1 antibody (10R-1299)

Availability: In stock

Price: $190.00
Size: 50 ug
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