c-Jun antibody (20R-1889)

Rabbit polyclonal c-Jun antibody

Synonyms Polyclonal c-Jun antibody, Anti-c-Jun antibody, JUN antibody, AP1 antibody, Phospho Thr93 antibody
Cross Reactivity Human, Mouse, Rat
Applications IHC, WB
Immunogen c-Jun antibody was raised in Rabbit using the peptide sequence around phosphorylation site of threonine 93 (T-P-T(p)-P-T) derived from Human c-Jun as the immunogen.

Images

Immunohistochemical staining using c-Jun antibody (20R-1889)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Phospho-Thr93) antibody (left) or the same antibody preincubated with with blocking peptide (right)

Specifications

Host Rabbit
Method of Purification c-Jun antibody was purified by immunoaffinity chromatography.
Form & Buffer Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC: 1:50-1:100, WB: 1:500-1:1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance c-Jun is the name of a gene and protein that, in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function. c-Jun knockout is lethal, but transgenic animals with a mutated c-Jun that cannot be phosphorylated (termed c-JunAA) can survive.

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Images

  • Immunohistochemical staining using c-Jun antibody (20R-1889) | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Phospho-Thr93) antibody (left) or the same antibody preincubated with with blocking peptide (right)
  • Western Blot analysis using c-Jun antibody (20R-1889) | Western Blot analysis of extracts from Hela cells untreated or treated with PMA using c-Jun (Phospho-Thr93) antibody

Availability: In stock

Price: $225.00
Size: 50 ug
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