CAD antibody (20R-2909)

Rabbit polyclonal CAD antibody

Synonyms Polyclonal CAD antibody, Anti-CAD antibody, DFFB antibody, DNA fragmentation factor antibody, 40kDa antibody, beta polypeptide (caspase-activated DNase) antibody, 40kDa antibody, BOS_15888 antibody, Cad antibody, caspase-activated deoxyribonuclease antibody, caspase-activated DNase antibody, caspase-activated nuclease antibody, CH211-147A11.2 antibody, CPAN antibody, DFF2 antibody, DFF40 antibody, DFF-40 antibody, Didff antibody, DNA fragmentation factor 40 kDa subunit antibody, DNA fragmentation factor antibody, 40 kD antibody, beta polypeptide antibody, DNA fragmentation factor 40 kD beta polypeptide (caspase-activated DNase) antibody, DNA fragmentation factor antibody, 40 kD antibody, beta polypeptide (caspase-activated DNase) antibody, DNA fragmentation factor antibody, 40 kD antibody, beta subunit antibody, DNA fragmentation factor antibody, beta polypeptide (caspase-activated DNase) antibody, DNA fragmentation factor antibody, beta subunit antibody, DNA fragmentation factor subunit beta antibody, DNA fragmentation factor subunit beta-like antibody, DNase inhibited by DNA fragmentation factor antibody, RP13-531C17.1 antibody, RP23-254N4.2 antibody
Cross Reactivity Human
Applications WB
Immunogen CAD antibody was raised in Rabbit using a synthetic target peptide as the immunogen

Images

Western Blot analysis using CAD antibody (20R-2909)

Specifications

Host Rabbit
Isotype IgG
Method of Purification CAD antibody was purified by Protein A affinity chromatography
Form & Buffer Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Usage & Assay Information

Usage Recommendations WB: 1:2000,

Storage & Safety

Storage Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles

General Information

Biological Significance CAD (caspase-activated DNase), a 40 kDa nuclear protein, is primarily responsible for cell-autonomous DNA degradation during apoptosis. CAD is present in healthy cells where it is held in an inactive state through the association with its inhibitor ICAD. The ICAD protein is inactivated in apoptotic cells via caspase-3 cleavage thereby releasing CAD, which subsequently cleaves chromosomal DNA. CAD is a magnesium-dependent endonuclease specific for double stranded DNA that generates double strand breaks with 3'-hydroxyl ends. The nuclease preferentially attacks chromatin in the internucleosomal linker DNA. However, the nuclease hypersensitive sites can be detected and CAD is potentially involved in large-scale DNA fragmentation as well. CAD-mediated DNA fragmentation triggers chromatin condensation that is another hallmark of apoptosis.

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Images

  • Western Blot analysis using CAD antibody (20R-2909) |

Availability: In stock

Price: $295.00
Size: 100 ul
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