CAPSL protein (His tag) (80R-1737)
Purified recombinant Human CAPSL protein
|Synonyms||Calcyphosine-like protein, protein|
Coomassie Blue stained SDS-PAGE of CAPSL protein (His tag) (80R-1737)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-208 amino acids: MGSSHHHHHH SSGLVPRGSH MAGTARHDRE MAIQAKKKLT TATDPIERLR LQCLARGSAG IKGLGRVFRI MDDDNNRTLD FKEFMKGLND YAVVMEKEEV EELFQRFDKD GNGTIDFNEF LLTLRPPMSR ARKEVIMQAF RKLDKTGDGV ITIEDLREVY NAKHHPKYQN GEWSEEQVFR KFLDNFDSPY DKDGLVTPEE FMNYYAGVSA SIDTDVYFII MMRTAWKL|
|Purity||> 90% pure|
|Molecular Weight||26.3 kDa (228aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||CAPSL, also known as Calcyphosine-like protein, is a calcium-binding protein containing two calcium-binding motifs (EF-hands). These conserved domains are found in a superfamily of calcium sensors and calcium signal modulators. Furthermore, the CAPSL gene is in the same linkage disequilibrium (LD) block as the IL7R gene. It was reported association between the CAPSL-IL7R locus and type 1 diabetes. Recombinant human CAPSL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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