Carbonic Anhydrase protein (His tag) (80R-1067)
Purified recombinant E.coli Carbonic Anhydrase protein
|Synonyms||Carbonate dehydratase protein, JW0122 protein, Car1 protein, CA1 protein, Carbonate dehydratase I protein, CAN protein, Carbonic anhydrase B., yadF protein, CA I protein, Carbonic anhydrase 1 protein, CA 1 protein, Car 1 protein, ECK0125 protein, CAI protein|
Coomassie Blue stained SDS-PAGE of Carbonic Anhydrase protein (His tag) (80R-1067)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-220 amino acids: MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK|
|Purity||> 95% pure|
|Molecular Weight||27 kDa (240aa), confirmed by MALDI-TOF.|
|Form & Buffer||Liquid in 20mM Tris pH8.0, 1mM DTTDTT, 10% glycerol|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O ? HCO3? + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E. coli and purified by conventional chromatography techniques.|
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