CARD12 antibody (70R-10429)

Affinity purified rabbit polyclonal CARD12 antibody

Synonyms Polyclonal CARD12 antibody, Anti-CARD12 antibody, CARD-12 antibody, CARD12, CARD-12, CARD 12, CARD 12 antibody
Cross Reactivity Human, Mouse, Rat
Applications IHC, WB
Immunogen CARD12 antibody was raised in rabbit using the C terminal of CARD12 as the immunogen
Assay Information CARD12 Blocking Peptide, catalog no. 33R-7636, is also available for use as a blocking control in assays to test for specificity of this CARD12 antibody

Images

Western blot analysis using CARD12 antibody (70R-10429)

Recommended NLRC4 Antibody Titration: 0.2-1 ug/ml

Specifications

Host Rabbit
Form & Buffer Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-1 ug/ml

Storage & Safety

Storage Store at 4 deg C, following reconstitution, aliquot and store at -20 deg C. Avoid repeated freeze-thaw cycles.

General Information

Biological Significance In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1, NOD1, and NOD2. These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.

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Images

  • Western blot analysis using CARD12 antibody (70R-10429) | Recommended NLRC4 Antibody Titration: 0.2-1 ug/ml
  • Immunohistochemical staining using CARD12 antibody (70R-10429) | Human Smooth Muscle

Availability: In stock

Price: $345.00
Size: 50 ug
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