CBFA2T2 antibody (10R-1199)

Mouse monoclonal CBFA2T2 antibody

Synonyms Monoclonal CBFA2T2 antibody, Anti-CBFA2T2 antibody, Core-binding factor runt domain alpha subunit 2 antibody, CBFAT 2, CBFAT-2 antibody, CBFAT-2, MTGR1 antibody, CBFAT 2 antibody, ZMYND3 antibody, CBFA2T2, EHT antibody
Specificity Human CBFA2T2
Cross Reactivity Human. Other species not studied
Applications Dot Blot, FC, ICC, WB
Immunogen CBFA2T2 antibody was raised in mouse using recombinant Core-Binding Factor,Alpha Subunit 2

Images

Flow Cytometric assay using CBFA2T2 antibody (10R-1199)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CBFA2T2 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone CBF5I134
Isotype IgG1
Method of Purification CBFA2T2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer CBFA2T2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.

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Images

  • Flow Cytometric assay using CBFA2T2 antibody (10R-1199) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CBFA2T2 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using CBFA2T2 antibody (10R-1199)
  • Immunofluorescent staining using CBFA2T2 antibody (10R-1199) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-CBFA2T2 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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